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12_Engineering the Human Gut Microbiome

ASSIGNMENT
(2a) Design a milli- or micro-fluidic 'artificial gut' or other 'organ-on-a-chip' device to be utilized, at a minimum, for cell culture. 
(2b) Fabricate your device, or at least one component of your device. 
What features of your organ are you attempting to emulate?
How is your device intended to function?
Were you able to fabricate your device? Which components? Which parts 'worked' and which ones didn't?
What will you aim to improve for your next iteration of design + build?
(2c) Culture the organism from (1) in your milli- or micro-fluidic device. 
(3) Share your 'final' device designs on 'Metafluidics'
Methodology
1> Brainstorming about how create structures through microfluidics. 
2> Designing and making the synthetic organ.
3> Testing. 
1> Brainstorming about how create structures through microfluidics.
6_BIOFAB_GLAND_1

Glands are fundamental components of the endocrine System.

The endocrine system, unlike the nervous system that sends electrical impulses, regulates various body functions (such as growth) by chemical / biological signals (hormones).  https://en.wikipedia.org/wiki/Endocrine_system 

Matter and information are traveling together to control the evolution of different  structures.

00_GLAND-toward-spinning-artificial-spider-silk-anna_rising-Jan-Johansson

Source: Toward spinning artificial spider silk. Anna Rising, Jan Johansson

Spider’s gland is one of the most famous organs in nature used to create structures, and also used for micro fluidics applications. HERE some interesting references.

(2a) Design a milli- or micro-fluidic ‘artificial gut’ or other ‘organ-on-a-chip’ device to be utilized, at a minimum, for cell culture.

First sketches about the artificial gland and its negative control.

01.0_GLAND

Displaying IMG-20151117-WA0018.jpgDisplaying IMG-20151117-WA0018.jpgIMG-20151117-WA0018 (2)

Artificial Gland | General System | First sketch | Beno.

GLAND

Artificial Gland

My goal is create a system to control the production of 3 differents materials: Keratin (strong and resistant material), Collagen (very flexible material) and Fibrin (Flexible and strong material).
The control system is through salinity level. It will stimulate the production of different inductors.
These inductors will control only specifics bacteria to produce our target proteins.

6_BIOFAB_GLAND_3

Reference: On the top of the picture: Surface tension and structures creation. T5 – FAU PUCP 2014. Faculty: B. Miller, A. Calmell del S., B. Juarez | Down > Artificial Gland device v 1.0 | HTGAA. Author: Beno Juarez , Lima 2015

01.1_GLAND

Artificial Gland | First Prototype. Components: 2mm acrylic, 3” diameter. Made with laser cutter. DXF File HERE.

02.1_GLAND

Differents materials. By the moment I’m working with chemical components. From left to right: Acid: Shampoo, Base: Yogurt, Red Acid (shampoo), Blue Acid (shampoo) and yelow Acid (shampoo). Next step, use bacterias.

PROCESS

Step 1: Put the base on the bottom.

Step 2: Put the colours and inyect the acid.

The Acid (shampoo) will react with the base (Yogurt) creating a fractal structure.

Digital view | Microscope

 

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